Depiction in the comic

Twilight and her friends in Equestria Girls

In My Little Pony Equestria Girls, Twilight and Spike teleport to a human world to retrieve the stolen element of magic from Sunset Shimmer. Twilight and Spike enter Canterlot High School and meet the human counterparts of their Ponyville friends. When Twilight discovers that Principal Celestia will award the crown to the Princess of the Fall Formal dance, she decides to run for Princess. She wins despite a smear campaign orchestrated by Sunset Shimmer, and receives the crown at the dance. However, Sunset Shimmer takes the crown by force and transforms into a rage-filled demonic creature. She hypnotizes the students, whom she plans to use as an army to invade Equestria. When she attacks Twilight, Twilight's friends shield her and inadvertently activate the magic of friendship. They defeat Sunset Shimmer, who is remorseful for her actions.
After the dance, Twilight and Spike return to Equestria. Twilight feels more confident about being a princess than before. She bumps into Flash Sentry, who she encountered several times in the human world, and blushes. Twilight's friends tease her for having a crush, but she denies it.